一株γ-聚谷氨酸合成菌的筛选与鉴定.PDF

微生物学通报 FEB 20, 2011, 38(2): 164−168 Microbiology China © 2011 by Institute of Microbiology, CAS tongbao@ 研究报告 一株 γ-聚谷氨酸合成菌的筛选与鉴定 1 1 1* 2 1 1 姚俊 陈宽婷 魏钦俊 阮文辉 曹新 鲁雅洁 (1. 南京医科大学基础医学院生物技术系 江苏 南京 210029) (2. 山西省医药与生命科学研究院 山西 太原 030001) 摘 要: 从土壤中筛选分离获得一株 γ-聚谷氨酸合成菌 PGS-1, 经鉴定为枯草芽孢杆菌(Bacillus subtilis), 在富含谷氨酸和葡萄糖的培养基中可大量合成 γ-聚谷氨酸, 摇瓶发酵产量达 26 g/L, 不 同于大多文献报道的微生物合成的γ-聚谷氨酸具有较高的分子量, 该菌株合成的 γ-聚谷氨酸分子 量较低(3×105−4×105 kD), 分子量分布较窄, 可适用于低分子量要求的应用领域, 如作为药物的控 缓释载体, 值得深入开发研究。 关键词: γ-聚谷氨酸, 筛选, 枯草芽孢杆菌, 摇瓶发酵 Screening and identification of a novel γ-polyglutamic acid producing strain YAO Jun1 CHEN Kuan-Ting1 WEI Qin-Jun1* RUAN Wen-Hui2 CAO Xin1 LU Ya-Jie1 (1. Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu 210029, China) (2. Shanxi Institute of Medicine and Life Science, Taiyuan, Shanxi 030001, China) Abstract: A novel γ-polyglutamic acid (γ-PGA) producing-strain Bacillus subtilis PGS-1 was isolated from soil, which could produced a large mount of γ-PGA in the medium containing glucose and gluta- mate. The highest yield 26 g/L of γ-PGA was obtained in flask culture. Compared with other reported γ-PGA producing-strain, γ-PGA derived from Bacillus subtilis PGS-1 had a lower molecular weig