HPLC法测定旋覆花药材中旋覆花素和去乙酰旋覆花素的含量_马立满.pdf

HPLC 娉曟祴瀹氭棆瑕嗚姳鑽潗涓棆瑕嗚姳绱犲拰鍘讳箼閰版棆瑕嗚姳绱犵殑鍚噺螖 锛 # 椹珛婊 锛屽垬 闇囷紝灏氭槑鑻憋紝鍒樺箍瀛︼紝寰 椋庯紝钄″皯闈掞紙鍖椾含澶у鑽闄㈢敓鑽鐮旂┒瀹わ紝鍖椾含 100191锛 涓浘鍒嗙被鍙 R284.1 鏂囩尞鏍囧織鐮 A 鏂囩珷缂栧彿 1001-0408锛?016锛?3-0369-03 DOI 10.6039/j.issn. 1001-0408.2016.03.28 鎽 瑕 鐩殑锛氬缓绔嬫祴瀹氭棆瑕嗚姳鑽潗涓棆瑕嗚姳绱犲拰鍘讳箼閰版棆瑕嗚姳绱犲惈閲忕殑鏂规硶銆傛柟娉曪細閲囩敤楂樻晥娑茬浉鑹茶氨娉曘€傝壊璋辨煴涓篫orbax SB-C18锛屾祦鍔ㄧ浉涓轰箼鑵?姘达紙姊害娲楄劚锛夛紝娴侀€熶负1.0ml/min 锛屾煴娓╀负25 鈩冿紝妫€娴嬫尝闀夸负210nm 锛岃繘鏍烽噺涓?0渭l銆傜粨鏋滐細鏃嬭鑺辩礌鍜屽幓 涔欓叞鏃嬭鑺辩礌鐨勬娴嬭川閲忔祿搴︾嚎鎬ц寖鍥村垎鍒负0.0002锝?.005銆?.000 1锝?.0017渭g/ml 锛坮 锛?.9998銆?.9994锛夛紱绮惧瘑搴︺€佺ǔ瀹氭€с€侀噸 澶嶆€ц瘯楠岀殑RSD 锛?锛咃紱鍔犳牱鍥炴敹鐜囧垎鍒负99.63锛咃綖103.56锛呫€?5.98锛咃綖101.21锛咃紝RSD 鍒嗗埆涓?.26锛呫€?.84锛咃紙n 锛?锛夈€傜粨璁猴細璇ユ柟 娉曟搷浣滅畝渚匡紝缁撴灉鍑嗙‘銆佸彲闈狅紝鍙敤浜庤瘎浠锋棆瑕嗚姳鑽潗鐨勮川閲忋€ 鍏抽敭璇 鏃嬭鑺憋紱鏃嬭鑺辩礌锛涘幓涔欓叞鏃嬭鑺辩礌锛涘惈閲忔祴瀹氾紱楂樻晥娑茬浉鑹茶氨娉 Simultaneous Determination of Inulicin and Deacetylinulicin in Inulae Flos by HPLC MA Liman 锛孡IU Zhen 锛孲HANG Mingying 锛孡IU Guangxue 锛孹U Feng 锛孋AI Shaoqing 锛圖ivision of Pharmacognosy 锛 School of Pharmacy 锛孭eking University 锛孊eijing 100191锛孋hina 锛 ABSTRACT OBJECTIVE 锛歍o establish a method of simultaneous determination of inulicin and deacetylinulicin in Inulae Flos. METHODS 锛欻PLC was performed on the column of Zorbax SB-C18 with mobile phase of acetonitrile-water 锛坓radient elution 锛塧t a flow rate of 1.0 ml/min 锛宼he column temperature was 25 鈩冿紝the detection wavelength was 210 nm 锛宎nd the injection volume was 10渭l. RESULTS 锛歍he linear range was 0.000 2-0.005 渭g/ml 锛坮 锛?.999 8锛塮or inulicin and 0.000 1-0.001 7 渭g/ml 锛坮 锛?.999 4锛塮or deacetylinulicin 锛汻SDs of precision 锛宻tability and reproducibility tests were lower than 2.0锛咃紱recoveries were 99.63锛?103.56锛 锛圧SD 锛?.26锛咃紝n 锛?锛塧nd 95.98锛?101.21锛咃紙RSD 锛?.84锛咃紝n 锛?锛夛紝respectively. CONCLUSIONS 锛歍he method is simple 锛宎ccurate and reliable 锛宎nd can be used for the quality evaluation of Inulae Flos. KEYWORDS Inulae Flos 锛汭nulicin 锛汥eacetylinulicin 锛汣ontent determination 锛汬PLC 鏃嬭鑺变负鑿婄妞嶇墿鏃ユ湰鏃嬭鑺憋紙Inul