the genome sequence of rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite立克次氏体的基因组序列猫属识别第一个假定的接合质粒的专性细胞内寄生虫.pdf

Open access, freely available online PLoS BIOLOGY The Genome Sequence of Rickettsia felis Identifies the First Putative Conjugative Plasmid in an Obligate Intracellular Parasite 1* 2 ´ 1 2 1 1,2 Hiroyuki Ogata , Patricia Renesto , Stephane Audic , Catherine Robert , Guillaume Blanc , Pierre-Edouard Fournier , 2 1 2* Hugues Parinello , Jean-Michel Claverie , Didier Raoult ´ ´ ´ 1 Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France, 2 Unite des Rickettsies, UMR 6020, IFR 48, CNRS, Faculte de Medecine, Marseille Cedex, France We sequenced the genome of Rickettsia felis, a flea-associated obligate intracellular a-proteobacterium causing spotted fever in humans. Besides a circular chromosome of 1,485,148 bp, R. felis exhibits the first putative conjugative plasmid identified among obligate intracellular bacteria. This plasmid is found in a short (39,263 bp) and a long (62,829 bp) form. R. felis contrasts with previously sequenced Rickettsia in terms of many other features, including a number of transposases, several chromosomal toxin–antitoxin genes, many more spoT genes, and a very large number of ankyrin- and tetratricopeptide-motif-containing genes. Host-invasion-related genes for patatin and RickA were found. Several phenotypes predicted from genome analysis were experimentally tested: conjugative pili and mating were observed, as well as b-lactamase activity, actin-polymerization-driven mobility, a