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a novel ring finger gene, sbrfp1, increases resistance to cold-induced sweetening of potato tubers论文.pdf

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FEBS Letters 587 (2013) 749–755 journal homepage: www.FEBSL A novel RING finger gene, SbRFP1, increases resistance to cold-induced sweetening of potato tubers Huiling Zhang, Xun Liu, Jun Liu, Yongbin Ou, Yuan Lin, Meng Li, Botao Song ⇑, Conghua Xie ⇑ Key Laboratory of Horticultural Plant Biology (HAU), Ministry of Education; National Centre for Vegetable Improvement (Central China); Huazhong Agricultural University, Wuhan 430070, China a r t i c l e i n f o a b s t r a c t Article history: The modulation of the activity of enzymes associated with carbohydrate metabolism is important Received 30 October 2012 for potato cold-induced sweetening (CIS). A novel RING finger gene SbRFP1 was cloned and its Revised 26 January 2013 expression was found to be cold-inducible in potato tubers of the CIS-resistant genotypes. Transfor- Accepted 28 January 2013 mation of SbRFP1 in potatoes confirmed its role in inhibiting b-amylase and invertase activity, which Available online 8 February 2013 consequently slowed down starch and sucrose degradation and the accumulation of reducing sug- Edited by Julian Schroeder ars in cold stored tubers. These findings strongly suggest that SbRFP1 may function as a negative reg- ulator of BAM1 and StvacINV1 to decelerate the accumulation of reducing sugars in the process of potato CIS. Keywords: Potato 2013 Federation of European Biochemical Societies. Published by Elsevier B.V.
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