基因芯片Expression of a novel bHLH-Zip gene in human testis.pdf

维普资讯 ShaJHetal／AsianJAndrol2003Jun；5{2)：83—88 ．83． @ 2003．AsianJournalofAndrology ISSN lOO8．682X ShanghaiInstituteofMateriaMedica ChineseAcademyofSciences ． http：Hwww．AsiaAndro．com ExpressionofanovelbHLH--Zipgeneinhumantestis Jia-HaoSHA ，Zuo—MinZHOU ’，Jian—MinLI，MingLIN HuiZHUz，HuZHU ，Ya—DongZHOU~， ， Li—LongWANCd，Yi—QuanWANG，Kai—YaZHOU Instituteofgeneticresources，NanjingnormalUniversity,Nanjing210029,China 。Keylaboratoryofreproductivemedicine，NanjingmedicalUniversiyt,Nanjing，210029，China Keywords：basichelix。-loop。-helix；leucinezipper；testis；spermatogenesis；DNA microarrays Abstract Aim：Toidentifyspecificallyexpressedgenesintheadultandfetaltestes ． M ethods：A humantestiseDNA microarraywasestablished．ThenthemRNA ofadultandfetaltestiswaspurifiedandprobeswerepreparedbya reversetranscriptionreactionwiththetestismRNA astemplate ． 1r}lemicroarraywashybridizedwiht probesofadult andfetaltestes．Thenucleicsequencesofdifferentiallyexpressedgenesweredeterminedandhomologieswere serachedinhtedatabasesofhteGenBank．Results： nhybridizedwithadultorfetaltestisprobe s ． htepositive cloneswere96．8％ and95．4％，respectively．Amongthesegenesonewasanew testis—specificgene．whichwas ， namedTSP1．TSP1washighlyexpressedinhumanadulttestis