基因芯片Application of DNA chip techniques for Yq microdeletion analysis in infertile males.pdf

EXPERIMENTAL and MOLECULAR MEDICINE, Vol. 36, No. 2, 179-184, April 2004 Application of DNA chip techniques for Yq m icrodeletion analysis in infertile m ales S u m a n L e e 1*, H y u n S u k J o o 2 * The application of DNA chip technology to identify S o o k -H w a n L e e 1 , J u n g E u n P a rk 2 Yq deletions can also facilitate our understanding J o n g M a n K im 2 , J a e H o o n H w a n g 2 of m ale infertility. K ey S eu n g C h o 2 an d S eu n g Y o n g H w an g 2,3 Keywords: DNA chip; male infertility; microdeletion; 1 molecular diagnosis; STS Genome Research Center for Reproductive Medicine and Infertility Infertility Medical Center, CHA General Hospital College of Medicine, Pochon CHA University Introduction Seoul 135-913, Korea 2 Department of Biochemistry MicroBiochip Center Y-chromosomal deletions represent the most frequent Hanyang University GenoCheck Co. Ltd. genetic alterations in infertile men (Chandley and Ansan, Gyeonggi-do 426-791, Korea Cooke, 1994). The frequency of deletions on the long 3Corresponding author: Tel, 82-31-400-5516; arm of the Y chromosome (Yq) in infertile male Fax, 82-31-502-5518; E-mail, syhwang@hanyang.ac.kr patients varies from 1% to 51% depending on patient *These authors contributed equally to this work. se