β-胡萝卜素合成的代谢工程研究进展.PDF

578 生 物 工 程 学 报 王岩岩 等/ β-胡萝卜素合成的代谢工程研究进展 Chinese Journal of Biotechnology /cjbcn April 25, 2017, 33(4): 578−590 DOI: 10.13345/j.cjb.160356 ©2017 Chin J Biotech, All rights reserved ·综 述· - 1 2 1 王岩岩 ，邢建民 ，陈红歌 1 450002 2 100190 , , . β-. , 2017, 33(4): 578–590. Wang YY, Xing JM, Chen HG. Progress in metabolic engineering of β-carotene synthesis. Chin J Biotech, 2017, 33(4): 578–590. 摘 要: β-胡萝卜素是自然界中最重要的商业化生产的植物色素之一，具有多种生理功能和生物活性。自上世 纪60 年代开始，随着系统生物学概念的提出以及对类胡萝卜素合成途径研究的不断深入，系统代谢工程在提 高类胡萝卜素产量方面发挥了重要作用。文中在介绍 β-胡萝卜素传统生产方法的基础上，重点介绍了如何运 用系统代谢工程手段构建 β-胡萝卜素高产菌株，并分析了进一步提高工程菌 β-胡萝卜素产量所面临的主要问 题及可能的解决方案，为 β-胡萝卜素的高效生产提供了思路。 : β- 胡萝卜素，前体物质，合成途径，系统代谢工程 Progress in metabolic engineering of β-carotene synthesis 1 2 1 Yanyan Wang , Jianmin Xing , and Hongge Chen 1 College of Life Sciences, Henan Agricultural University , Zhengzhou 450002, Henan, China 2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering , Chinese Academy of Sciences, Beijing 100190, China Abstract: β-carotene is an important natural plant pigment and has various physiological functions in organisms. With the proposition of systematic biology and progress in carotenoids biosynthesis since the 1960s, metabolic engineering has played a significant role in enhancing carotenoid production. In this review, we present β-carotene’s traditional production methods and metabolic engineering strategies for constructing β-carotene-producing strains. Meanwhile, mai