assessment of the antiviral properties of recombinant porcine sp-d against various influenza a viruses in vitro评估的抗病毒特性重组猪sp-d针对各种流感病毒在体外.pdf

Assessment of the Antiviral Properties of Recombinant Porcine SP-D against Various Influenza A Viruses In Vitro 1 2 1 1 3,4 Marine L. B. Hillaire , Martin van Eijk , Stella E. van Trierum , Debby van Riel , Xavier Saelens , 5 5 1 1 1,6 Roland A. Romijn , Wieger Hemrika , Ron A. M. Fouchier , Thijs Kuiken , Albert D. M. E. Osterhaus , Henk P. Haagsman2*, Guus F. Rimmelzwaan1,6* 1 Erasmus Medical Centre, Rotterdam, The Netherlands, 2 Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands, 3 Department for Molecular Biomedical Research, VIB, Ghent, Belgium, 4 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium, 5 U-Protein Express B.V., Science Park Utrecht, Utrecht, The Netherlands, 6 Viroclinics Biosciences BV, Rotterdam, The Netherlands Abstract The emergence of influenza viruses resistant to existing classes of antiviral drugs raises concern and there is a need for novel antiviral agents that could be used therapeutically or prophylacticaly. Surfactant protein D (SP-D) belongs to the family of C- type lectins which are important effector molecules of the innate immune system with activity against bacteria and viruses, including influenza viruses. In the present study we evaluated the potential of recombinant porcine SP-D as an antiviral agent against influenza A viruses (IAVs) in vitro. To determine the range of antiviral activity, thirty IAVs of the subtypes H1N1, H3N2 and H5N1 that originated from birds, pigs