cross-protective potential of a novel monoclonal antibody directed against antigenic site b of the hemagglutinin of influenza a virusescross-protective潜在的新型单克隆抗体针对抗原site b的a型流感病毒的血凝素.pdf

Cross-Protective Potential of a Novel Monoclonal Antibody Directed against Antigenic Site B of the Hemagglutinin of Influenza A Viruses 1 1 2 3 1 Reiko Yoshida , Manabu Igarashi , Hiroichi Ozaki , Noriko Kishida , Daisuke Tomabechi , Hiroshi 1,4 1 1 Kida , Kimihito Ito , Ayato Takada * 1 Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Hokkaido, Japan, 2 Department of Veterinary Microbiology, Faculty of Agriculture, Tottori University, Tottori, Japan, 3 Laboratory of Influenza Viruses, Department of Virology III, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan, 4 Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan Abstract The hemagglutinin (HA) of influenza A viruses has been classified into sixteen distinct subtypes (H1–H16) to date. The HA subtypes of influenza A viruses are principally defined as serotypes determined by neutralization or hemagglutination inhibition tests using polyclonal antisera to the respective HA subtypes, which have little cross-reactivity to the other HA subtypes. Thus, it is generally believed that the neutralizing antibodies are not broadly cross-reactive among HA subtypes. In this study, we generated a novel monoclonal antibody (MAb) specific to HA, designated MAb S139/1, which showed heterosubtypic cross-reactive neutralization and hemagglutination inhibition of influenza A viruses. This MAb was found to have broad reactivity to many other viruses (H1, H2, H3, H5, H9, and H13 subtypes) in enzyme-linked immunosorbent assays. We further found that MAb S139/1