snailase preparation of ginsenoside m1 from protopanaxadiol-type ginsenoside and their protective effects against ccl4-induced chronic hepatotoxicity in mice从protopanaxadiol-type snailase制备人参皂苷m1人参皂苷和对老鼠ccl4-induced慢性肝毒性的保护作用.pdf

Molecules 2011, 16, 10093-10103; doi:10.3390/molecules161210093 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Snailase Preparation of Ginsenoside M1 from Protopanaxadiol-Type Ginsenoside and Their Protective Effects Against CCl -Induced Chronic Hepatotoxicity in Mice 4 Wei Li 1,2,†, Ming Zhang 1,†, Yi-Nan Zheng 2, Jing Li 1, Ying-Ping Wang 3, Yun-Jing Wang 1, Jian Gu 1, Ying Jin 4, Hui Wang 4,* and Li Chen 1,* 1 Norman Bethune College of Medicine, Jilin University, Changchun 130021, China 2 College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China 3 Institute of Special Wild Economic Animals and Plant, CAAS, Jilin 132109, China 4 China-Japan Union Hospital, Jilin University, Changchun 130033, China † These authors contributed equally to this work and were considered co-first authors. * Authors to whom correspondence should be addressed; E-Mails: wanghu (H.W.); zhouweichen@ (L.C.); Tel.: +86-0431-8561-9922. Received: 21 October 2011; in revised form: 24 November 2011 / Accepted: 29 November 2011 / Published: 6 December 2011 Abstract: To investigate the protective effects of protopanaxadiol-type ginsenoside (PDG) and its metabolite ginsenoside M1 (G-M1) on carbon tetrachloride (CCl )-induced chronic 4 liver injury in ICR mice, we carried out conversion of protopanaxadiol-type ginsenosides to ginsenoside M1 using snailase. The optimum time for the conversi