social isolation-induced aggression potentiates anxiety and depressive-like behavior in male mice subjected to unpredictable chronic mild stress社会isolation-induced侵略强化焦虑和雄性老鼠则行为受到不可预知的慢性温和应激.pdf

Social Isolation-Induced Aggression Potentiates Anxiety and Depressive-Like Behavior in Male Mice Subjected to Unpredictable Chronic Mild Stress 1. 1. 1 1 1 2 1,2 Xian-cang Ma , Dong Jiang , Wen-hui Jiang , Fen Wang , Min Jia , Jin Wu , Kenji Hashimoto , Yong- 3 1 hui Dang *, Cheng-ge Gao * 1 Department of Psychiatry, First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xian, China, 2 Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Chiba, Japan, 3 Key Laboratory of Environment and Genes Related to Diseases of the Education Ministry, Key Laboratory of the Health Ministry for Forensic Medicine, Department of Forensic Medicine, Xi’an Jiaotong University School of Medicine, Xi’an, China Abstract Background: Accumulating epidemiological evidence shows that life event stressors are major vulnerability factors for psychiatric diseases such as major depression. It is also well known that social isolation in male mice results in aggressive behavior. However, it is not known how social isolation-induced aggression affects anxiety and depressive-like behavior in isolated male mice subjected to unpredictable chronic mild stress (CMS), an animal model of depression. Methodology/Principal Findings: C57/B6 male mice were divided into 3 groups; non-stressed controls, in Group I; isolated mice subjected to the CMS protocol in Group II and aggression by physical contact in socially isolated mice subjected to the CMS protocol in Group III. In the sucrose intake test, ingestion of a 1% sucrose solution by mice in Groups II and III was significantly lower than in Group I. Furthermore, intake of this solution