antibody dynamics of 2009 influenza a (h1n1) virus in infected patients and vaccinated people in china抗体动态2009年甲型流感(h1n1)病毒感染的病人和接种疫苗的人在中国.pdf

Antibody Dynamics of 2009 Influenza A (H1N1) Virus in Infected Patients and Vaccinated People in China Ming Wang*., Jun Yuan., Tiegang Li., Yang Liu, Jibin Wu, Biao Di, Xi Chen, Xinhong Xu, Enjie Lu, Kuibiao Li, Yanhui Liu, Yejian Wu, Xiongfei Chen, Peng He, Yulin Wang, Jianhua Liu Guangzhou Center for Disease Control and Prevention, Guangzhou, People’s Republic of China Abstract Background: To evaluate the risk of the recurrence and the efficiency of the vaccination, we followed-up antibody responses in patients with the 2009 pandemic H1N1 influenza and persons who received the pandemic H1N1 vaccine in Guangzhou China. Methods: We collected serum samples from 129 patients and 86 vaccinated persons at day 0, 15, 30, 180 after the disease onset or the vaccination, respectively. Antibody titers in these serum samples were determined by haemagglutination inhibition (HI) assay using a local isolated virus strain A/Guangdong Liwan/SWL1538/2009(H1N1). Results: HI antibody positive rate of the patients increased significantly from 0% to 60% at day 15 (x2 = 78, P,0.001) and 100% at day 30 (x2 = 23, P,0.001), but decreased significantly to 52% at day 180 (x2 = 38, P,0.001), while that of vaccinated subjects increased from 0% to 78% at day 15 (x2 = 110, P,0.001) and 81% at day 30 (x2 = 0.32, P = 0.57), but decreased significantly to 34% at day 180 (x2 = 39, P,0.001). Geometric mean titers (GMT) of HI antibodies in positive samples from the patients did not change significantly between day 15 and day 30 (T = 0.92, P = 0.36), but it decreased significantly from 80 at day 30 to 52 at day 180 (T = 4.5, P,0.001). GMT of vaccinated persons increased significantly from 100 at day 15 to 193 at day 30 (T = 4.5, P,0.001), but deceased significantly to 74 at day 180 (T = 5.1, P,0.001). Compared to the patients, the vaccinat