antibody complementarity-determining regions (cdrs) a bridge between adaptive and innate immunity抗体complementarity-determining地区(cdrs)之间的一座桥梁的适应性和先天免疫.pdf

Antibody Complementarity-Determining Regions (CDRs): A Bridge between Adaptive and Innate Immunity 1. 1. 1 1 2 2 Elena Gabrielli , Eva Pericolini , Elio Cenci , Federica Ortelli , Walter Magliani , Tecla Ciociola , 1 2 1 2 Francesco Bistoni , Stefania Conti , Anna Vecchiarelli *, Luciano Polonelli 1 Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy, 2 Microbiology Section, Department of Pathology and Laboratory Medicine, University of Parma, Parma, Italy Abstract Background: It has been documented that, independently from the specificity of the native antibody (Ab) for a given antigen (Ag), complementarity determining regions (CDR)-related peptides may display differential antimicrobial, antiviral and antitumor activities. Methodology/Principal Findings: In this study we demonstrate that a synthetic peptide with sequence identical to V CDR3 H of a mouse monoclonal Ab (mAb) specific for difucosyl human blood group A is easily taken up by macrophages with subsequent stimulation of: i) proinflammatory cytokine production; ii) PI3K-Akt pathway and iii) TLR-4 expression. Significantly, VHCDR3 exerts therapeutic effect against systemic candidiasis without possessing direct candidacidal properties. Conclusions/Significance: These results open a new scenario about