花椰菜ACC氧化酶基因的克隆及其RNAi对内源基因表达的抑制作用.pdf

Genetica 遗传学报Acta Sinica，July2005，32(7)：764—769 SSN0379—4172 ofACCOxidaseGeneand Cloning Inhibitionof Gene I in withithRNAi。nRNA Expression Exl： CHEN Yin—Hual，ZHANG Zhi．Bia01，2’① Jun-Hon91，OUYANGB01’2，LIHan-Xia2，YE of Genetic (1．NationalLaboratoryCrop Key Improvement，Wuhan430070，China； of Horticultureand 2．Department AgriculturalUniversity，Wuhan430070，China) Forestry，Huazhong of1202 ofthecandidateAC0 was the Abstract：Afragment frOm cauliflowerfbrassicaoler- bp gene amplified acea the whichwere totheconsensusse— Var．botrytis)genomedegeneratedprimers using designedaccording ofACOaminoacids various resultofBLASTshowedthe quence among plantspecies．The sequence presented ave matchwiththeACO fromother ry wasfrOm83％to99％．Threeexons high genes plants：thehomologue andtwointronswereidentifiedinthis ofmRNAwas ntand sequence．The 756 encoded252ami． splicedlength noacids．The new wasdenominated submittedto the putativegene BoACO，and constructedanRNA