微悬臂生物传感器检测生物素-亲和素相互作用.pdf

2011 年第69 卷 化 学 学 报 Vol. 69, 2011 第2 期, 243～246 ACTA CHIMICA SINICA No. 2, 243～246 ·研究简报· 微悬臂生物传感器检测生物素-亲和素相互作用 张慧勇a,b 潘宏青a,b 唐纪琳*,a 张柏林*,a (a 中国科学院长春应用化学研究所电分析化学国家重点实验室 长春 130022) (b 中国科学院研究生院 北京 100049) 摘要 将生物素共价键合到微悬臂的硅表面, 利用微悬臂传感技术在流动注样条件下研究了生物素-亲和素的动态作 用过程, 同时利用原子力显微镜(AFM)力刻蚀技术验证了生物素化硅表面结合亲和素的有效性. 结果显示亲和素与生 物素作用产生较大的表面应力驱动微悬臂偏转, 悬臂偏转的程度和速率与亲和素的浓度相关, 亲和素浓度越大, 微悬 臂偏转程度也越大, 同时反应速率也更快. 这项工作为开发新型的无标记检测技术, 研究生物分子之间相互作用提供 了有益的探索. 关键词 微悬臂; 生物素; 亲和素; 相互作用 Microcantilever Biosensor on the Biotin-avidin Interaction Zhang, Huiyonga,b Pan, Hongqinga,b Tang, Jilin*,a Zhang, Bailin*,a a ( State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sci- ences, Changchun 130022) (b Graduate University of Chinese Academy of Sciences, Beijing 100049) Abstract The dynamic interaction process of biotin-avidin was investigated by biotin covalently modified microcantilever sensor in real-time in a flowing cell. The specific interaction was confirmed by the evidence that the avidin layer can be mechanically removed with atomic force microscopy nanolithography. The de- flection of the modified cantilever increased with concentration of avidin. Microcantilever biosensor pro- vides a good method to explore novel label-free technology and monitor various interactions between bio- molecules. Keywords microcantilever; biotin; avidin