studies on the interactions of copper and zinc ions with β-amyloid peptides by a surface plasmon resonance biosensor研究铜和锌离子的相互作用与β-amyloid多肽表面等离子体共振生物传感器.pdf

Int. J. Mol. Sci. 2012, 13, 11832-11843; doi:10.3390/ijms130911832 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Studies on the Interactions of Copper and Zinc Ions with β-Amyloid Peptides by a Surface Plasmon Resonance Biosensor Fujun Yao 1, Ruiping Zhang 2,*, He Tian 2 and Xiangjun Li 1,* 1 College of Chemistry and Chemical Engineering, Graduate University, Chinese Academy of Sciences, Beijing, 100049, China; E-Mail: yaofuj09b@ 2 Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China; E-Mail: tianhe@ * Authors to whom correspondence should be addressed; E-Mails: rpzhang@ (R.Z.); lixiangj@ (X.L.); Tel.: +86-10-6316-5218 (R.Z.); +86-10-8825-6336 (X.L.); Fax: +86-10-6316-5218 (R.Z.); +86-10-8825-6093 (X.L.). Received: 18 July 2012; in revised form: 7 September 2012 / Accepted: 7 September 2012 / Published: 19 September 2012 Abstract: The aggregation of β-amyloid peptide (Aβ) into fibrils plays an important role in the pathogenesis of Alzheimer’s disease (AD). Metal ions including copper and zinc are closely connected to the precipitation and toxicity of Aβ. In this study, a surface plasmon resonance (SPR) biosensor was constructed to investigate the interactions between Aβ and metal ions. Aβ peptide was immobilized on the SPR chip surface through a preformed alkanethiol self-assembled monolayer (SAM).