surface plasmon spectroscopic detection of saxitoxin表面等离子体光谱贝类毒素的检测.pdf

Sensors 2007, 7, 1216-1223 sensors ISSN 1424-8220 © 2007 by MDPI /sensors Communication Surface Plasmon Spectroscopic Detection of Saxitoxin 1 2 3 4 4 Hongxia Chen , Youn Sook Kim , Sam-Rok Keum , Sung-Hoon Kim , Heung-Jin Choi , Jaebeom Lee 2, Won Gun An 2 and Kwangnak Koh 2, * 1 College of Pharmacy, Pusan National University, Pusan 609-735, Korea 2 College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea 3 College of Science and Technology, Korea University, Choong-nam 339-700, Korea 4 College of Engineering, Kyungpook National University, Taegu 702-701, Korea * Author to whom correspondence should be addressed; E-mail: koh@pusan.ac.kr Received: 18 June 2007 / Accepted: 13 July 2007 / Published: 16 July 2007 Abstract: For the surface-optoelectronic study of Saxitoxin sensing, we fabricated self- assembled calix[4]arene derivative monolayers as the recognition-functional interfaces on a gold surface. An interaction study between Saxitoxin and calix[4]arene derivative monolayers were performed using surface plasmon resonance (SPR) spectroscopy. Among three calix[4]arene derivatives, calix[4]arene crown ether SAM showed the highest sensitivity to Saxitoxin. The detection limit of this system is three orders of magnitude lower than that of the mouse bioassay which