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细胞培养基本知识(Cell culture basic knowledge).doc

发布:2017-07-21约1.41万字共12页下载文档
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细胞培养基本知识(Cell culture basic knowledge) 1 cell culture Cell culture is an important technology in biomedicine, and it is widely applied in various fields such as cell biology, biochemistry and clinical testing. 1.1 source of cells It can be directly from human or animal tissues or indirectly from cell lines. The former is directly from the organism, and the biological indexes are closer to the body. However, the extraction process of normal cells is more complicated, and most cells can only be grown or can be transmitted from one to two generations, such as human vascular endothelial cells and mouse nerve cells. The advantages of cell line are more convenient, which can be purchased directly and used without separation process. Generally, it can be passed on a stable way, but its biological indicators are different from those in the body. 1.2 laboratory requirements for cell culture Cell culture requires high cell culture, with the aim of enabling cells to survive in aseptic conditions. The surgical instruments that extract the cells should be sterilized with high temperature and high pressure before each use, and the water of the mixed culture medium is required to be used to double steam water and join the double resistance. It is better to use the culture bottle, centrifuge tube and other utensils. To operate in a sterile room. Before each operation, use the ultraviolet lamp to illuminate the table 20-30min, then wash the hands clean, change the slippers into the sterile room, and use the new jure to extinguish the operation. Endothelial cells of human vascular endothelial cells were used to describe cell culture Because of the different nature of the cell, the separation and cultivation methods can be different. Its origin is usually fresh umbilical cord of umbilical vein (from) within 24 h after delivery, the separation of the specific method is: take neonatal umbilical cord blood (25 cm), and into the green, streptomycin each 100 u/mL of sterilization in PBS, 4
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