人核迁移蛋白单克隆抗体的制备及其在人巨核细胞中的表达.pdf

894 !墨塑!塑!=墨Z≥墨绁照皇筮王鱼瘗堂盘查(￡垡坠』g趔丛型!堡里坠磐122鲤!：丝f!) ·论著· 人核迁移蛋白单克隆抗体的制备及其在人巨核细胞中的表达 陈彦球1，葛怡琛2，杨 510275；3中山大学公共卫生学院，广东广州510008；) ofthemonoclonal [Keywords]hNudC；monoclonal Preparation antibody humanhNudCandits 【摘要】 目的：制备人核迁移蛋白(hNudC)的单克隆抗体 against expres- sioninhuman (mAb)并鉴定其特性。方法：从人胎肝组织中克隆得到 megakaryocytes CHEN Yan3宰 Yan．qiul，GEYi—chen2，YANG FirstAffdiated Yat·sen Hospital，SunUniversity，Guan孕hou 用免疫荧光染色法及Westernblot试验鉴定mAb的特异性。 510008．Chi胞 AIM：To andcharacterizethemon· [Abstract] prepare oclonal humannucleardistribution anybody(mAb)against ofhNudCwas X C(hNudC)．METHODS：Thecodingregion 1：l×105和1：5 fromhuman livertissueandtherecombi- amplified embryo 性反应，免疫荧光染色和Westernblot试验证明，制备的mAb nant vector wasconstructed． prokaryoticexpressionpET28b hNudC was asafusion anN- proteinexpressed protein、fI，ith terminal6-His recombinanthNudC tag．Thepurified protein制备了特异性抗hNudCmAb，为研究hNudC蛋白的功能、天 mice mAb． WasusedtoimmunizeBALB／c for prepanng 然分布及异常表达奠定了基础。 The cells frommABwerescreened hybddomaprocuced by C关键词】人核迁移蛋白；单克隆抗体；巨核细胞 ELISAandthe ofmAbwas munohis— specificity analyzedby 【中图分类号]R392．11[文献标识码】B tochemicalandWestern staining blot．RESULTS：Twoby- bndoma and mAb hNudC cells(2C162D8)secrtingagainst nucleardistribution 人核迁移蛋白C基因(human were ofthetwomAIDswere developed．The isotypes IgG! that titer and detectionsho