介绍一种简易高效的大鼠原代肝细胞分离方法.pdf

20 11 2 34 1 BM U Journ a,l Feb. 20 11, V o.l 34, N o. 1 # 29# * 1 1 1 1 2 张兴元 宋向芹 张 芳 欧 琨 吕 毅 1 25660 3; 2 = 介绍 一种简易高效的大鼠原代肝细胞分离方法 在 Se lgen 两步胶原酶灌注法基础上加 改进, 进行 大鼠原代肝细胞分离 平均每只成年 SD 大鼠获取 ( 1. 56 ? 0. 31) @ 108 个肝细胞, 平均肝细胞活率 ( 95. 1 ? 4. 2) % , 平 均肝细胞纯度 9 8% 本试验介绍方法简便易行, 且肝细胞产量高活力好纯度高, 适宜在 一般条件实验室推广应用 = 关键词: 原代肝细胞; 分离; 胶原酶 = R 329. 2 = A = 1001-95 10 ( 2011) 0 1-0029-04 In troduce a fac ility and h igh efficien cy separat ion m ethod of rat p rim ary hepatocytes 1 1 1 1 2 ZHANG X ngyuan SONG X angq n ZHANG F ang OU K un LB Y 1 A ff l a ted H osp ta l o f B nzhou M ed cal U n vers ty, B nzhou 256603; 2 the F rstA ff l ated H osp ta l of X an J aotong U n vers ty = A b stract O bj ective Introduce a fac l ty and h gh e ff c ency sepa rat on m e thod of rat prm ary hepa to cy tes. M eth ods R a t hepato- cy tes w e re so la ted and pur f ed by us ng the m od f ed Selgen. s wt o-step m ethod. R esults T he average y eld of rat hepa tocy tes w as ( 1156 ? 0. 31) @ 108 percen t rat, w th an average v ab l ty o f ( 95. 1 ? 4. 2) % and pur ty of 98% . Conc lusions The sepa ra t on m eth- od of hepatocytes w as sm ple and easy, w th adv antages o f h gh y eld, good v ab l ty and h gh pur ty, so t w ou ld be appl ed w dely n o r- d nary laboratory. = K ey w ords P rm ary hepatocy tes; Sepa ra