synthesis of β-maltooligosaccharides of glycitein and daidzein and their anti-oxidant and anti-allergic activities合成β-maltooligosaccharides glycitein和大豆苷及其抗氧化和抗过敏的活动.pdf

Molecules 2010, 15, 5153-5161; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Synthesis of β-Maltooligosaccharides of Glycitein and Daidzein and their Anti-Oxidant and Anti-Allergic Activities Kei Shimoda 1,* and Hiroki Hamada 2,* 1 Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan 2 Department of Life Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama 700-0005, Japan * Authors to whom correspondence should be addressed; E-Mails: shimoda@med.oita-u.ac.jp (K.S.); hamada@dls.ous.ac.jp (H.H.); Tel. +81-97-586-5606 (K.S.); +81-86-256-9473 (H.H.); Fax: +81-97-586-5619 (K.S.); +81-86-256-8468 (H.H.). Received: 10 June 2010; in revised form: 25 July 2010 / Accepted: 27 July 2010 / Published: 29 July 2010 Abstract: The production of β-maltooligosaccharides of glycitein and daidzein using Lactobacillus delbrueckii and cyclodextrin glucanotransferase (CGTase) as biocatalysts was investigated. The cells of L. delbrueckii glucosylated glycitein and daidzein to give their corresponding 4- and 7-O-β-glucosides. The β-glucosides of glycitein and daidzein were converted into the corresponding β-maltooligosides by CGTase. The 7-O-β- glucosides of glycitein and daidzein and 7-O-β-maltoside of glycitein showed inhibitory effects on IgE antibody production. On the other hand, β-glucosides of glycitein an