药学毕业论文--维药刺山柑叶子中微量元素和氨基酸成分的研究.doc
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维药刺山柑叶子中微量元素和氨基酸成分的研究
【摘要】 目的分析测定维药刺山柑叶子中的矿质元素和氨基酸成分。方法采用火焰分光光度法和原子吸收分光光度法分析测定了刺山柑叶子13种矿质元素, 采用日立835-50型氨基酸自动分析仪测定了叶子中的氨基酸成分。结果刺山柑叶子富含K,Ca,Mg,Na 4种常量元素和Fe,Zn,Mn,Cu,Ni,Co 6种微量元素, 重金属元素Pb,Cr的含量均低于国家标准。刺山柑叶子中富含18种氨基酸成分,其中包括7种人体必需氨基酸,总氨基酸含量为17.88 %。结论维药刺山柑叶子富含矿质元素和氨基酸,具有潜在的开发价值。
【关键词】 刺山柑 微量元素 氨基酸
Study on the Components and Contents of the Trace Element and Amino Acid in the Leaves of Capparis spinosa
Abstract:ObjectiveTo determine the constituents of trace elements and amino acids in the leaves of Capparis spinosa L.MethodsCompositions and contents of K and Na were determined by flame spectrophotometry, others were determined by atomic absorption spectrophotometer Model PE800. Amino acid was determined by Hitachi automatic amino acid analyzer Model 835-50.ResultsThe leaves of Capparis spinosa contained 13 species of elements, including 4 species of microelements, 6 species of trace elements and 3 species of weight elements. Except for Cd, the contents of heavy metal Pb and Cr were much lower than the national standards of food hygiene. The leaves of Capparis spinosa contained 18 species of amino acids, including 7 species of essential amino of human acid. Total amino acids contents were amounted for 17.88%. ConclusionThe leaf of Capparis spinosa contain rich microelements and amino acids,which reserved to be further exploited.
Key words:Leaf of Capparis spinosa L.; Trace element; Amino acid
刺山柑Capparis spinosa L.系山柑科山柑属藤本植物,俗称“槌果藤”,其果实称为“老鼠瓜”“苦瓜”“野西瓜”,维语称为“波里克果” 或“卡盘”,是荒漠、干旱地区极有栽培价值的抗旱植物,我国新疆各沙区分布较广,以吐鲁番盆地沙漠最多[1]。其根、叶、果可入药,维吾尔族民间常用其鲜果捣碎贴敷治疗关节炎和肩周炎等疾病。国外对其药理作用也进行了研究[2],国内外已有其果实化学成分的研究报道[3~6],但其叶子中化学成分的研究未见报道。为了评价其营养价值,为进一步探索其药理作用和研究开发该植物资源奠定基础,本实验采用火焰分光光度法、原子吸收分光光度法、氨基酸分析仪对刺山柑叶子中矿质元素和氨基酸成分进行了分析测定。
1 器材
1.1 材料刺山柑植物样品于200607采自新疆库车戈壁滩,经塔里木大学植物科技学院李志军教授鉴定为山柑科山柑属植物刺山柑Capparis spinosa L.。刺山柑叶子经高温杀青、阴干、粉碎、过筛备用。
1.2 仪器K,Na火焰分光光度计,PE800型原子吸收分光光度计,日立835-50型氨基酸自动分析仪,管式自控电热消化器,Direct-Q3型Milipore超纯水系统。
1.3 试剂HNO3(GR),HClO4(GR),超纯水,18种氨基酸标准品和13种待测元素的标准品(AR),浓硫酸、甲醇、
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