the flavonoid luteolin inhibits fcγ-dependent respiratory burst in granulocytes, but not skin blistering in a new model of pemphigoid in adult mice类黄酮的毛地黄黄酮抑制fcγ-dependent粒细胞呼吸爆发,但不是皮肤起泡在成年老鼠类天疱疮的新模式.pdf

The Flavonoid Luteolin Inhibits Fcc-Dependent Respiratory Burst in Granulocytes, but Not Skin Blistering in a New Model of Pemphigoid in Adult Mice 1,2,4 2 2 ¨ 3 2,5,6 Eva Oswald , Alina Sesarman , Claus-Werner Franzke , Ute Wolfle , Leena Bruckner-Tuderman , 1 1 2,5 Thilo Jakob , Stefan F. Martin , Cassian Sitaru * 1 Allergy Research Group, Department of Dermatology, University Freiburg Medical Center, Freiburg, Germany, 2 Molecular Dermatology, Department of Dermatology, University Freiburg Medical Center, Freiburg, Germany, 3 Competence Centre Skintegral, Department of Dermatology, University Freiburg Medical Center, Freiburg, Germany, 4 Faculty of Biology, University of Freiburg, Freiburg, Germany, 5 BIOSS Centre for Biological Signalling Studies, Freiburg, Germany, 6 Freiburg Institute for Advanced Studies, Freiburg, Germany Abstract Bullous pemphigoid is an autoimmune blistering skin disease associated with autoantibodies against the dermal-epidermal junction. Passive transfer of antibodies against BP180/collagen (C) XVII, a major hemidesmosomal pemphigoid antigen, into neonatal mice results in dermal-epidermal separation upon applying gentle pressure to their skin, but not in spontaneous skin blistering. In addition, this neonatal mouse model precludes treatment and observation of diseased animals beyond 2– 3 days. Therefore, in the present study we have developed a new disease model in mice reproducing the spontaneous blistering and the chronic course characteristic of the human condition. Adult mice were pre-immunized with rabbit IgG followed by