cre-dependent expression of multiple transgenes in isolated neurons of the adult forebraincre-dependent表达多种转基因在孤立的前脑神经元的成年.pdf

Cre-Dependent Expression of Multiple Transgenes in Isolated Neurons of the Adult Forebrain 1 2 1 1 3 Sridhara Chakravarthy , Tara Keck , Martijn Roelandse , Robin Hartman , Andreas Jeromin , Sean 1 2¤ 2¤ 1 Perry , Sonja B. Hofer , Thomas Mrsic-Flogel , Christiaan N. Levelt * 1 Molecular Visual Plasticity Group, Netherlands Institute for Neuroscience, Amsterdam, The Netherlands, 2 Department of Cellular and Systems Neurobiology, Max Planck Institute of Neurobiology, Martinsried, Germany, 3 Allen Institute for Brain Science, Seattle, Washington, United States of America Abstract Background: Transgenic mice with mosaic, Golgi-staining-like expression of enhanced green fluorescent protein (EGFP) have been very useful in studying the dynamics of neuronal structure and function. In order to further investigate the molecular events regulating structural plasticity, it would be useful to express multiple proteins in the same sparse neurons, allowing co-expression of functional proteins or co-labeling of subcellular compartments with other fluorescent proteins. However, it has been difficult to obtain reproducible expression in the same subset of neurons for direct comparison of neurons expressing different functional proteins. Principal Findings: Here we describe a Cre-transgenic line that allows reproducible expression of transgenic proteins of choice in a small number of neurons of the adult cortex, hippocampus, striatum, olfactory bulb, subiculum, hypothalamus, superior colliculus and amygdala. We show that using these Cre-transgenic mice, multiple Cre-dependent transgenes can be expressed toget