包头泉山金矿浸矿酸性微生物群落优势度变化的比较研究-微生物学报.PDF
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微生物学通报 Jan. 20, 2014, 41(1): 43−49
Microbiology China © 2014 by Institute of Microbiology, CAS
tongbao@ DOI: 10.13344/j.microbiol.china.130087
研究报告
包头泉山金矿浸矿酸性微生物群落优势度变化的比较研究
聂立 税刘杨 王建英*
( 014010)
摘 要:【目的】研究不同矿石组成对微生物群落结构的影响。【方法】选取包头泉山金矿的酸
性矿坑水(Acid mine drainage ,AMD)对4 种不同组成的矿石样品进行浸矿,采用 16S rRNA-PCR
和RFLP 相结合的方法,研究浸矿前后浸矿微生物种群优势度的变化情况。【结果】所选取的3
个酸性矿坑水考查点之间浸矿微生物的种类及数量相似度较大,浸矿微生物结构相对变化度不
大。H71 和F11 相对其他两个样品的群落结构差异较大,而J72 和S71 几乎一样,说明在钾长
型和石英型矿石的选择压力下,浸矿微生物的被选择趋势是大致相同的。【结论】浸矿前后样
品中浸矿微生物的系统发育分析结果表明,所考查的克隆子的序列可分为两大分支:
Acidithiobacillus 菌属和一个相对独立的菌属,且这两个分支内部菌株之间的发育距离较近。
关键词:AMD ,优势群落,浸矿微生物,重金属浸出
Comparison of bacterial communities in the Baotou Quanshan gold
before and after acid leaching
*
NIE Li SHUI Liu-Yang WANG Jian-Ying
(School of Mathematics, Physics and Biological Engineering, Inner Mongolia University of Science and Technology,
Baotou , Inner Mongolia 014010, China)
Abstract: [Objective] Influence on bacterial communities of different ore composition. [Methods]
The change of acidic microbial community dominance before and after leaching ore were investi-
gated by using 16S rRNA-RFLP methods. [Results] AMD of three sampling points are the inde-
pendent Acidithiobacillus bacteria genera of microbes, which accounts for about half the number of
tested strains, three sample points between ore leaching microbial species and quantity larger simi-
larity. Meantime, we
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