pGEX-5X-1大肠杆菌表达载体说明.pdf

pGEX-5X-1 编号 载体名称 北京华越洋生物VECT4470 pGEX-5X-1 pGEX-5X-1 载体基本信息 别名: pGEX-5X-1, pGEX5X1, pGEX 5X 1 质粒类型: 大肠杆菌蛋白表达载体 表达水平: 高拷贝 启动子: Tac 克隆方法: 多克隆位点，限制性内切酶 载体大小: 4972 bp 5 测序引物及序列: pGEX5: GGGCTGGCAAGCCACGTTTGGTG 3 测序引物及序列: pGEX3: CCGGGAGCTGCATGTGTCAGAGG 载体标签: N-GST 载体抗性: Ampicillin 氨苄 备注: 复制子是pMB1 产品目录号: 27-4854-01 稳定性: 瞬时表达 Transient 组成型: 诱导表达 病毒/非病毒: 非病毒 pGEX-5X-1 载体质粒图谱和多克隆位点信息 pGEX-5X-1 载体简介 There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sep