assessing protein immunogenicity with a dendritic cell line-derived endolysosomal degradome评估与树突细胞免疫原性的蛋白质line-derived endolysosomal degradome.pdf

Assessing Protein Immunogenicity with a Dendritic Cell Line-Derived Endolysosomal Degradome 1 ¨ 2 1 2 3 4 Matthias Egger *, Alexander Jurets , Michael Wallner , Peter Briza , Silke Ruzek , Stefan Hainzl , Ulrike 2 ¨ 5 5 3 ´ 1 Pichler , Claudia Kitzmuller , Barbara Bohle , Christian G. Huber , Fatima Ferreira 1 Christian Doppler Laboratory for Allergy Diagnosis and Therapy, Department of Molecular Biology, University of Salzburg, Salzburg, Austria, 2 Department of Molecular Biology, Division of Allergy and Immunology, University of Salzburg, Salzburg, Austria, 3 Department of Molecular Biology, Division of Chemistry and Bioanalytics, University of Salzburg, Salzburg, Austria, 4 Laboratory for Immunological and Molecular Cancer Research, Paracelsus Private Medical University of Salzburg, Salzburg, Austria, 5 Christian Doppler Laboratory for Immunomodulation, Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria Abstract Background: The growing number of novel candidate molecules for the treatment of allergic diseases imposed a dramatic increase in the demand for animal experiments to select immunogenic vaccines, a pre-requisite for efficacy. Because no in vitro methods to predict the immunogenicity of a protein are currently available, we developed an in vitro assay that exploits the link between a protein’s immunogenicity and its susceptibility to endolysosomal proteolysis. Methodology: We compared protein composition and proteolytic activity of endolysosomal fractions isolated from murine bone marrow- and human blo