cord blood derived cd4+cd25high t cells become functional regulatory t cells upon antigen encounter脐带血中cd4 + t细胞cd25high成为功能性调节性t细胞抗原相遇.pdf

+ high Cord Blood Derived CD4 CD25 T Cells Become Functional Regulatory T Cells upon Antigen Encounter 1 1 1 2 3 2 Elisabeth Mayer , Christina Bannert , Saskia Gruber , Sven Klunker , Andreas Spittler , Cezmi A. Akdis , ´ 1. 1,2 . Zsolt Szepfalusi , Thomas Eiwegger * 1 Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria, 2 University of Zurich, Swiss Institute of Allergy and Asthma Research (SIAF), Davos, Switzerland, 3 Surgical Research Laboratories and Core Facility Flow Cytometry, Medical University of Vienna, Vienna, Austria Abstract Background: Upon antigen exposure, cord blood derived T cells respond to ubiquitous environmental antigens by high proliferation. To date it remains unclear whether these ‘‘excessive’’ responses relate to different regulatory properties of the putative T regulatory cell (Treg) compartment or even expansion of the Treg compartment itself. Methods: Cord blood (.37 week of gestation) and peripheral blood (healthy controls) were obtained and different Treg cell subsets were isolated. The suppressive potential of Treg populations after antigen exposure was evaluated via functional inhibition assays ([3H]thymidine incorporation assay and CFSE staining) with or without allergen stimulation. The frequency and markers of CD4+CD25highFoxP3+ T cells were characterized by mRNA analysis and flow cytometry. Results: Cord blood derived CD4+