DNA barcoding of genus Toxoptera Koch （Hemiptera： Aphididae） Identification and molecular phylogeny inferred from mitochondrial COl sequences.pdf

InsectScience(2009)16，475—484，DOI10．1111．1744—7917．2009．01270x DNAbarcodingofgenusToxopteraKoch(Hemiptera： Aphididae)Identificationandmolecularphylogenyinferred frOm mitochondrialCOlsequences Jian-FengWang ，2andGe—XiaQiao’ InstituteofZoology,ChineseAcademyofSciences，Being，and CoflegeofBiologicalandEnvironmentalEngineering,Shenyang University,Shenyang,China Abstract Identiticationofaphidspeciesisalwaysdifficultduetotheshortageofeasily distinguishablemorphologicalcharacters．AphidgenusToxopteraconsistsofspecieswith similarmorphologyand similartoAphisinmostmorphologicalcharactersexceptthe stridulatoryaDparatus．DNA barcodeswith 1145bpsequencesofpartialmitochondrial cytochrome．coxidaseI(CO1、geneswereusedforaccurateidentificationofToxoptera． Resultsindicatedmean intraspecificsequencedivergenceswere 1．33％．whereasmean interspecificdivergencesweregreaterat8．29％ f0．13％ and7．79％ ifT．aurantii3andT． aurantii4arecrypticspecies)．Sixteensamplesweredistinguishedtofourspeciescorrectly byCOIbarcodes．whichimpliedthatDNA barcodingwassuccessfu1indiscriminationof aphidspecieswithsimilarmorphology．Phylogeneticrelationshipsamongspeciesofthis genusweretestedbasedonthisportion otCoIsequences．FourspeciesofToxoptera assembledacladewithlow supportinmaximum—parsimonyfMP1analysis．maximum． 1ikelihood(ML)analysisandBayesianphylogenetictrees，thegenusToxopterawasnot monophyletic．andthereweretwosistergroups。suchas citricidusand victoriae．and