Dynamic distribution and stem cell characteristics of Sox1-expressing cells in the cerebellar cortex.pdf

CellResearch(2009)19：1324-1333． @2009IBCB．SIBS，CAS Allrightsreserved1001·06020／9$32．00 ORIGlNALARTICLE Dynam licdIistrIibUuttlioonn aand stem cellIIcharacteristIiCcSS of Soxl--expressingcellsinthecerebellarcortex JoelleAlcock ，VirginieSottile lWolfsonCentre)CorStemCells,TissueEngineeringandModelling(STEM)UniversityPark—CBSbuilding,Universit)’ofNotting- ham，Nottingham NG72RD,UnitedKingdom BergmanngliacellsareadiscreteradialgliapopulationsurroundingPurkinjecellsinthecerebeHarcortex．AI— thoughBergmanngliaareessentialforthedevelopmentandcorrectarborizationofPurkinjecells，littleisknown abouttheregulationofthiscellpopulationafterthedevelopmentalphase．Inanefforttocharacterizethispopulation atthemolecularlevel，wehaveanalyzedmarkerexpressionandestablishedthatadultBergmanngliaexpressSoxl， Sox2andSox9，afeatureotherwiseassociatedwithneuralstemcells(NSCs)．Inthepresentstudy,wehavefurther analyzedthedevelopmentalpatternofSoxl—expressingcellsinthedevelopingcerebellum．W ereportthatbeforebe- comingrestrictedtothePurkinjecelllayeKSoxl-positivecellsarepresentthroughouttheimmaturetissue，andthat thesecellsshow characteristicsofBergmanngliaprogenitors．OurstudyshowsthattheseprogenitorsexpressSox1． Sox2andSox9，asignaturemaintainedthroughoutcerebellarmaturationintoadulthood．W henisolatedinculture， theSoxl—expressingcerebellarpopulationexhibitedneurosphere—formingability,NSC-markercharacteristics，and demonstratedmultipotencyattheclonalleve1．Ourresultsshow thattheBergmanngliapopulationexpressesSoxl durin~cerebellardevelopment．andthatthesecellscanbeisolatedandshow stem cellcharacteristicsinvitro，sug— gestingthattheycouldholdabroaderpotentialthanpreviouslythought． Keywords：cerebellum，Soxgenes，stem cell C ffResearch(2009)19：1324—1333．doi：10．1038／cr．2009．119：publishedonline13October2009 IntrOducti0n