高分子材料研究方法-凝胶色谱法.ppt

GPC 1860~1930: porous materials have adsorption-separation function (especially for dye) 1940~1970: appearance of Liquid Chromatography 1964: The first GPC produced by Waters company 1970: HPLC （rapid test 、good separation effects、less sample、more sensitive）; New technology of HPLC applied in GPC area Mobile phase in GPC Gel in GPC Choice of gel (pore diameter) Column in GPC GPC系统之温度控制 Flow separation theory: very high flow velocity 单分散性标准曲线 Universal calibration method  普适标准曲线 6.3 Data Treatment Gel Chromatograms Correction Curve of Molecular Weight Calculation of DMW Correction of Width of Peak 6.3.1 Gel Chromatograms Plots: Horizontal axis: the retention values of the effluent ∝ logarithm of MW Vertical axis: concentration of eluent Monodispersive samples: Gaussian distribution curve Polydispersive samples: sum of distribution curves of monodispersive samples 6.3.2 Correction Curve of  Molecular Weight Keys: Transition from VR to MW Correction curve of molecular weight: lgM-V 6.3.2 Correction Curve of  Molecular Weight Direct Methods: Correction by monodispersive standards Exclusion limit / permeation limit Asymptote method: correction curves for wide distribution samples Hydrodynamics volume Retention volume Flexibility of macromolecule 流体力学体积与保留体积的关系曲线具有普适性，称为普适标准曲线 [?]=2.5NA?Vh/M [?]?M= 2.5NA?Vh [?]1?M1= [?]2?M2 [?] ： 特性黏数 NA ：阿伏伽德罗常数 Vh ：聚合物链等效球 的流体力学体积 K、?为每一特定高分子-溶剂体系的常数 Narrow-disperse Ps as guide sample 6.4 Applications on Polymers Applications in Production Copolymers Branching Degree Measurement of Materials of Low Molecules Aging Process 6.4.1 Applications in  Production 5.4.1 Inverse Gas  Chromatography Crystallinity and Crystalization Kinetics Crystallinity Crystalization Kinetics: Xc-t 6.4.2 Copolymers Hypenation with other methods 6.4.2 Copolymers Double Detectors 6.4.4 Measurement of  Materials of Low