禽呼肠孤病毒σA基因酵母双杂交诱饵载体的构建与鉴定.pdf

2017 44 3 847853 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j A A A GBKT A S RNA RT PCR A p GBKT GBKT A p p YHGold GBKT A p A A S A Construction and Identification of Bait Vector of Avian Reovirus A Gene in Yeast Twohybrid System LU Heng XIE Zhixun XIE Lii HUANG Li HUANG Jiaoling WANG Sheng j Z HANG Yanfang ZENG Tingting FAN Qing LUO Sisi XIE Zhi in DENG Xianw en q College of A nimal Science and Technology Guangx i Univ ersity N anning China Guangx i K ey L aboratory of A nimal Vaccines and New Technology Guangx i Veterinary Research Institute N anning China Abstract In order to screen the host protein that interacted w ith A gene of avian reovirus the bait vector pGBK T A of avian reovirus A gene in yeast tw ohy brid sy stem w as constructed T otal RN A of avian reovirus S standard strain w as purified by using T rizol reagent s Avian reovirus A gene w as amplified by RT PCR and w as cloned into yeast tw o hy brid bait vector pG BK T T he recombinant bait vector w as named as pGBK T A T he recombinant bait vector w as transf ormed into S accharomy ces cerev is iae YHGold A nd then the recombinant bait vector of