Y-ligation-based block shuffling - Protein Engineering Design and ...文档.pdf

Protein Engineering vol.15 no.10 pp.843–853, 2002 Construction of block-shuffled libraries of DNA for evolutionary protein engineering: Y-ligation-based block shuffling 1 1 Koichiro Kitamura , Yasunori Kinoshita , ing has attained, that of protein engineering has a long way Shinsuke Narasaki1 2 1 , Naoto Nemoto , Yuzuru Husimi and to go, partly because the former can be easily evolved as the Koichi Nishigaki1,3 same molecule can work for both function and information, 1 thus making the evolution of it much simpler and rapid (Wright Department of Functional Materials Science, Saitama University, 255 Shimo-Okubo, Saitama 338-8570 and 2GenCom Co., 11 Minami-Oya, and Joyce, 1997). The higher complexity of proteins is also a Machida 194-8511, Japan reason for this delayed progress. However, it is now clear that 3To whom correspondence should be addressed. the activities performed by RNA molecules are limited and E-mail: koichi@fms.saitama-u.ac.jp usually fall far short of that of the equivalent protein as a K.Kitamura and Y.Kinoshita contributed equally to this work trade-off for simplicity (Illangasekare et al., 1995). Therefore, Evolutionary protein engineering is now proceeding to a it is now time to develop further the technology for directed new stage in which novel technologies, besides the conven- evolution of proteins, and presented here is a st