composition, diversity, and origin of the bacterial community in grass carp intestine组合、多样性和草鱼肠道内细菌社区的起源.pdf

Composition, Diversity, and Origin of the Bacterial Community in Grass Carp Intestine 1 1 2 1 1 1 Shangong Wu , Guitang Wang *, Esther R. Angert , Weiwei Wang , Wenxiang Li , Hong Zou 1The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, and State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People’s Republic of China, 2 Department of Microbiology, Cornell University, Ithaca, New York, United States of America Abstract Gut microbiota has become an integral component of the host, and received increasing attention. However, for many domestic animals, information on the microbiota is insufficient and more effort should be exerted to manage the gastrointestinal bacterial community. Understanding the factors that influence the composition of microbial community in the host alimentary canal is essential to manage or improve the microbial community composition. In the present study, 16S rRNA gene sequence-based comparisons of the bacterial communities in the grass carp (Ctenopharyngodon idellus) intestinal contents and fish culture-associated environments are performed. The results show that the fish intestinal microbiota harbors many cellulose-decomposing bacteria, including sequences related to Anoxybacillus, Leuconostoc, Clostridium, Actinomyces, and Citrobacter. The most abundant bacterial operational taxonomic units (OTUs) in the grass carp intestinal content are those related to feed digestion. In addition, the potential pathogens and probiotics are important members of the intestinal microbiota. Fur