芍药苷的水解动力学研究.pdf

L ISH IZHEN MED I INE AND MATER IA MEDI A RESEAR H 2010 VOL. 21 NO. 3 2010 21 3 1 2 1* 1 刘 倩 , 刘永刚, 李继安 , 喇万英 ( 1. , 063000; 2. , 100102) : 考察芍药苷在不同 pH条件下的水解情况 D iamonsil- 18色谱柱( 250 mm 4. 6 mm, 5 m); 流动相为 - 1 乙腈 - 0. 1% 的甲酸水溶液( 15 85); 检测波长为 230 nm; 进样量为 10 l;柱温为室温, 流速为 1. 0 m l! m in 芍 药苷对照品在 0. 021 5~ 0. 215 g范围内, 进样量与峰面积间呈良好的线性关系, r= 0. 999 9,并得到不同 pH 条件下的水 解动力方程 芍药苷的水解符合一级动力学模型在不同 pH 条件下, 芍药苷的水解速度不同 : 芍药苷; 水解动力学; 高效液相色谱 : R284 : A : 2010) Study on Hydrolysis K inetics of Paeoniflorin 1 2 1* 1 LIU Q ian , LIU Yonggang , LI J ian , LA W anying ( 1.N orth China CoalM edical University, Tangshan 063000, China; 2. College of Traditional ChinesePharma cology, Beijing University of Traditional ChineseM edicine, Beij ing 100102, China) Abstract: Objective To investigate the hydrolysis kinetics ofPaeoniflorin in different pH values. M ethods D iamonsil- 18 col umn( 250mm 4. 6mm, 5m) w as used w ith the use of the acetonitriler- 0. 1% form ic acid ( 15 85) as the mobile phase, the flow rate was 1. 0m l! m in- 1 and the detection wavelengthw as set at 230 nm. Results Paeoniflorinw asw ell separated, the linear range of paeoniflorinw as 0. 021 5~ 0. 215g ( r= 0. 999 9). Hydrolysis kinetics equations in different pH valueswas obtained. Conclusion The degradation follow s pseudofirst- order kinetics. Hydrolysis rate is different in various pH values. Key words:Paeoniflorin; HydrolysisK inetics; HPL Paeonia lactfi lora Pal.l , 20. 0 ml, 100 m l, , [ 1] , , 10 l,, , ( Paeoniflorin), Y= 29747. 81 + 6. 892, r= 0. 999 9, [ 2] , 0.