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User Guide ® Amicon Ultra-15 10K Centrifugal Filter Devices for volumes up to 15 mL UFC901008 UFC901024 UFC901096 C V English Introduction Amicon® Ultra-15 10K centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Typical processing time is 15 to 40 minutes. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipettor, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging-bucket or fixed-angle rotor. Amicon® Ultra-15 10K devices are supplied non-sterile and are for single use only. Intended Use The Amicon® Ultra-15 product line includes 5 different cutoffs (Molecular Weight Cutoff, MWCO), however, the Amicon® Ultra-15 10K device (10,000 MWCO) is the only device intended for in vitro diagnostic use. It can be used to concentrate serum, urine, cerebrospinal fluid, and other body fluids prior to analysis. For information on other Amicon® Ultra-15 cutoffs, go to and enter Amicon Ultra-15 in the search box. Amicon® Ultra-15 10K Centrifugal Filter Devices 1 Applications ● Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples ● Purification of macromolecular components found in tissue culture extracts and cell lysates, r