crystallisation of wild-type and variant forms of a recombinant plant enzyme β-d-glucan glucohydrolase from barley (hordeum vulgare l.) and preliminary x-ray analysis结晶的野生型和变体形式的重组植物酶β-d-glucan glucohydrolase来自大麦(大麦l .)和初步的x射线分析.pdf

Int. J. Mol. Sci. 2010, 11, 2759-2769; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Crystallisation of Wild-Type and Variant Forms of a Recombinant Plant Enzyme β-D-Glucan Glucohydrolase from Barley (Hordeum vulgare L.) and Preliminary X-ray Analysis Sukanya Luang 1, James R. Ketudat Cairns 1, Victor A. Streltsov 2 and Maria Hrmova 3,* 1 School of Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand; E-Mail: jeab_bc@ (S.L.); cairns@sut.ac.th (J.R.K.C.) 2 Molecular and Health Technologies, CSIRO-Commonwealth Scientific Research Organization, Victoria 3052, Australia; E-Mail: victor.streltsov@csiro.au 3 Australian Centre for Plant Functional Genomics, University of Adelaide, Waite Campus, Glen Osmond, South Australia 5064, Australia * Author to whom correspondence should be addressed; E-Mail: maria.hrmova@.au; Tel.: +61-8-830-372-80; Fax: +61-8-830-371-02. Received: 28 May 2010; in revised form: 16 July 2010 / Accepted: 16 July 2010 / Published: 19 July 2010 Abstract: Wild-type and variant crystals of a recombinant enzyme β-D-glucan glucohydrolase from barley (Hordeum vulgare L.) were obtained by macroseeding and cross-seeding with microcrystals obtained from native plant protein. Crystals grew to dimensions of up to 500  250  375 µm at 2