wild-type phosphoribosylpyrophosphate synthase (prs) from mycobacterium tuberculosis a bacterial class ii prs野生型phosphoribosylpyrophosphate合成酶(prs)结核分枝杆菌细菌类ii prs.pdf

Wild-Type Phosphoribosylpyrophosphate Synthase (PRS) from Mycobacterium tuberculosis: A Bacterial Class II PRS? 1,2 1,2 1 1,2 Ardala Breda , Leonardo K. B. Martinelli , Cristiano V. Bizarro , Leonardo A. Rosado , 1,2 ´ 1,2 1,2 Caroline B. Borges , Diogenes S. Santos *, Luiz A. Basso * ˆ ´ ˜ 1 Instituto Nacional de Ciencia e Tecnologia em Tuberculose (INCT-TB), Centro de Pesquisas em Biologia Molecular e Funcional (CPBMF), Programa de Pos-Graduac¸ao em ´ ´ ´ ˜ Biologia Celular e Molecular, Pontifıcia Universidade Catolica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil, 2 Programa de Pos-Graduac¸ao em ´ ´ Biologia Celular e Molecular, Pontifıcia Universidade Catolica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil Abstract The 5-phospho-a-D-ribose 1-diphosphate (PRPP) metabolite plays essential roles in several biosynthetic pathways, including histidine, tryptophan, nucleotides, and, in mycobacteria, cell wall precursors. PRPP is synthesized from a-D-ribose 5- phosphate (R5P) and ATP by the Mycobacterium tuberculosis prsA gene product, phosphoribosylpyrophospha