complement and the alternative pathway play an important role in lpsd-galn-induced fulminant hepatic failure补充和替代途径lpsd-galn-induced暴发性肝衰竭中发挥重要作用.pdf

Complement and the Alternative Pathway Play an Important Role in LPS/D-GalN-Induced Fulminant Hepatic Failure 1. 1. 1 1 1 1 1 2 Shihui Sun , Yan Guo , Guangyu Zhao , Xiaojun Zhou , Junfeng Li , Jingya Hu , Hong Yu , Yu Chen , 3 4 5 5 5 4 Hongbin Song , Fei Qiao , Guilian Xu , Fei Yang , Yuzhang Wu , Stephen Tomlinson , Zhongping 2 1 Duan *, Yusen Zhou * 1 State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China, 2 Beijing You-An Hospital, Artificial Liver Center, Capital University of Medical Sciences, Beijing, China, 3 Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing, China, 4 Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina, United States of America, 5 Institute of Immunology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China Abstract Fulminant hepatic failure (FHF) is a clinically severe type of liver injury with an extremely high mortality rate. Although the pathological mechanisms of FHF are not well understood, evidence suggests that the complement system is involved in the pathogenesis of a variety of liver disorders. In the present study, to investigate the role of complement in FHF, we examined groups of mice following intraperitoneal injection of LPS/D-GalN: wild-type C57BL/6 mice, wild-type mice treated with a C3aR antagonist, C5aR monoclonal antibody (C5aRmAb) or CR2-Factor H (CR2-fH, an inhibitor of the alternative pathway), and C3 deficient mice (C32/ 2 mice). The animals were euthanize