基因组测序技术及其应用研究进展-安徽农业科学.PDF
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,J.AnhuiAgric.Sci.2018,46(22):2022,25
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ResearchProgressandApplicationofGenomeSequencingTechnology
LIGuozhi,DENGWeidong (YunnanAgricultureUniversity,Kunming,Yunnan650201)
Abstract GenomesequencingtechnologyhasbeendevelopedfromthefirstgenerationofSangersequencingtothethirdgenerationofsingle
moleculesequencingthroughthesecondgenerationofhighthroughputsequencing.Thesecondgenerationofhighthroughputsequencingtech
nologywasthemostcommonlyusedtechnologyingenomesequencing.Theapplicationprinciples,stepsandstatusofhighthroughputsequen
cingtechnologyingenomewidedenovosequencing,genomewideresequencing,reducedrepresentationsequencing,metagenomicsequencing
andepigeneticresearchwerereviewedinthispapertoprovidereferenceforapplyinggenomesequencingtechnology.
Keywords Highthroughputsequencing;Genomewideresequencing;Metagenomicsequencing;Epigenetic
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