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基因组测序技术及其应用研究进展-安徽农业科学.PDF

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- ,J.AnhuiAgric.Sci.2018,46(22):2022,25      ,   ( , 650201)   1 Sanger 2 3 , 2 。 denovo 、 、 、 、 , 。   ; ; ; - - -  Q75   A  2018 ResearchProgressandApplicationofGenomeSequencingTechnology LIGuozhi,DENGWeidong (YunnanAgricultureUniversity,Kunming,Yunnan650201) Abstract GenomesequencingtechnologyhasbeendevelopedfromthefirstgenerationofSangersequencingtothethirdgenerationofsingle moleculesequencingthroughthesecondgenerationofhighthroughputsequencing.Thesecondgenerationofhighthroughputsequencingtech nologywasthemostcommonlyusedtechnologyingenomesequencing.Theapplicationprinciples,stepsandstatusofhighthroughputsequen cingtechnologyingenomewidedenovosequencing,genomewideresequencing,reducedrepresentationsequencing,metagenomicsequencing andepigeneticresearchwerereviewedinthispapertoprovidereferenceforapplyinggenomesequencingtechnology. Keywords Highthroughputsequencing;Genomewideresequencing;Metagenomicsequencing;Epigenetic    Sanger 1 , , 。 1000bp, 99.99%, 。 DNA , , , , 。Roche/ , ,
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