contribution of herpesvirus specific cd8 t cells to anti-viral t cell response in humans贡献的疱疹病毒特异性cd8 t细胞在人类抗病毒t细胞反应.pdf

Contribution of Herpesvirus Specific CD8 T Cells to Anti- Viral T Cell Response in Humans 1 2 2 1 3 4 Elena Sandalova , Diletta Laccabue , Carolina Boni , Anthony T. Tan , Katja Fink , Eng Eong Ooi , 4 1,4 2 1,4 Robert Chua , Bahar Shafaeddin Schreve , Carlo Ferrari , Antonio Bertoletti * 1 Singapore Institute for Clinical Sciences, A*STAR, Singapore, 2 Unit of Infectious Diseases and Hepatology, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy, 3 Singapore Immunology Network, A*STAR, Singapore, 4 Emerging Viral Diseases, Duke – NUS Graduate Medical School, Singapore Abstract Herpesviruses infect most humans. Their infections can be associated with pathological conditions and significant changes in T cell repertoire but evidences of symbiotic effects of herpesvirus latency have never been demonstrated. We tested the hypothesis that HCMV and EBV-specific CD8 T cells contribute to the heterologous anti-viral immune response. Volume of activated/proliferating virus-specific and total CD8 T cells was evaluated in 50 patients with acute viral infections: 20 with HBV, 12 with Dengue, 12 with Influenza, 3 with Adenovirus infection and 3 with fevers of unknown etiology. Virus-specific (EBV, HCMV, Influenza) pentamer+ and total CD8 T cells were analyzed for activation (CD38/HLA-DR), proliferation (Ki-67/Bcl- 2low) and cytokine production. We observed that all acute viral infections trigger an expansion of activated/proliferating CD8 T cells, which differs in size depending on the infection but is invariably inflated by CD8 T cells specific for persistent her