基于生物质谱技术的磺酸化修饰策略及其在蛋白质组学中的应用.pdf

28 3 Vol. 28 No. 3 2007 8 Journal of Chinese Mass Spectromet ry Society Aug . 2007 赵丽艳, 周春喜, 张养军, 蔡 耘, 钱小红 ( , 100850) : , , y , , , , : ; ; ; : Q503; O657. 63 : A :2007) 0318508 Sulfonation Strategy Based on Mass Spectrometry and Its Application in Proteomics Research ZHAO Liyan, ZHOU Chunxi, ZHANG Yangjun, CAI Yun, QIAN Xiaohong ( I ns t it ute of R ad iat ion M ed ic ine, A cademy of M ilitar y M ed ical S ciences, B e ij ing 100850, Ch ina) Abstract: Negativelycharged sulfonic group introduced by sulfonation derivatization not on ly induced the M S/ M S fragmentation of peptides and produced an exclusive series of ytype ion spectrum, but also changed the charge distribution of sulfonated peptides and made the enrichment of target peptides by ionexchange chromatography possible. This techni ue e normously facilitates de novo peptide se uencing and protein identification by mass spec trometry. T he procedure of sulfonation derivatization and its application in the study of pro teomics are review ed. Key words: sulfonation; chemical modification; proteomics; biologic mass spectrometry , , Edman , [ 12] , : : : ( 2004CB520802) , ( , ( ( H030230280190) : ( 1980~ ) , ( ) , , , Email: zly_06@ 126. com : ( 1955~ ) , ( ) , , ,Email: ianxh @ nic. bmi. ac. cn 186 28 [ 3] [ 19] , , MALDI ( ladder , se uencing) [ 4] ( peptide , mass fingerprinting, PM F) [ 56] N C ( pos