slow unfolding of monomeric proteins from hyperthermophiles with reversible unfolding缓慢的单体的蛋白质从超嗜热菌可逆展开.pdf

Int. J. Mol. Sci. 2009, 10, 1369-1385; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Review Slow Unfolding of Monomeric Proteins from Hyperthermophiles with Reversible Unfolding Atsushi Mukaiyama 1 and Kazufumi Takano 2, 3, * 1 Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan; E-Mail: atsushi@ims.ac.jp 2 Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3 CREST, JST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan * Author to whom correspondence should be addressed; E-Mail: ktakano@mls.eng.osaka-u.ac.jp; Tel. +81-6-6879-4157; Fax: +81-6-6879-4157 Received: 28 January 2009; in revised form: 19 March 2009 / Accepted: 23 March 2009 / Published: 24 March 2009 Abstract: Based on the differences in their optimal growth temperatures microorganisms can be classified into psychrophiles, mesophiles, thermophiles, and hyperthermophiles. Proteins from hyperthermophiles generally exhibit greater stability than those from other organisms. In this review, we collect data about the stability and folding of monomeric proteins from hyperthermophilies with reversible unfolding, from the equilibrium and kinetic aspects. The results indicate that slow unfolding is a general strategy by whi