the actinome of dictyostelium discoideum in comparison to actins and actin-related proteins from other organisms盘基网柄菌的actinome discoideum相比,从而和actin-related蛋白质与其他生物.pdf

The Actinome of Dictyostelium discoideum in Comparison to Actins and Actin-Related Proteins from Other Organisms 1 2 1 3 1 Jayabalan M. Joseph , Petra Fey , Nagendran Ramalingam , Xiao I. Liu , Meino Rohlfs , Angelika A. 4 ¨ 1 ¨ 5 1 Noegel , Annette Muller-Taubenberger , Gernot Glockner , Michael Schleicher * 1 Adolf Butenandt Inst./Cell Biology and Center for Integrated Protein Science (CIPSM), Ludwig-Maximilians-University, Muenchen, Germany, 2 dictyBase, Center for Genetic Medicine, Northwestern University, Chicago, Illinois, United States of America, 3 Department of Biology II, Ludwig-Maximilians-University, Muenchen, Germany, 4 Institute for Biochemistry I, Center for Molecular Medicine Cologne (CMMC) and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Koeln, Germany, 5 Leibniz-Institute for Age Research - Fritz Lipmann Institute, Jena, Germany Abstract Actin belongs to the most abundant proteins in eukaryotic cells which harbor usually many conventional actin isoforms as well as actin-related proteins (Arps). To get an overview over the sometimes confusing multitude of actins and Arps, we analyzed the Dictyostelium discoideum actinome in detail and compared it with the genomes from other model organisms. The D. discoideum actinome comprises 41 actins and actin-related proteins. The genome contains 17 actin genes which most likely arose from consecutive gene duplications, are all active, in some cases developmentally regulated and coding for identical proteins (Act8-group). According to published data, the actin fraction in a D. discoideum cell consists of more than