survivin regulates the expression of vegf-c in lymphatic metastasis of breast cancer生存素调节vegf-c的表达在乳腺癌淋巴转移.pdf

Cai et al. Diagnostic Pathology 2012, 7:52 /content/7/1/52 RESEARCH Open Access Survivin regulates the expression of VEGF-C in lymphatic metastasis of breast cancer 1 1 1 1,2 3 1,2* Xiaopeng Cai , Shuai Ma , Ming Gu , Cong Zu , Wenzhi Qu and Xinyu Zheng Abstract Background: As a known regulator of apoptosis, survivin has positive relationship with lymphatic metastasis in breast cancer. This study aims to detect the difference in expression between survivin and vascular endothelial growth factor-C (VEGF-C) in treated breast cancer cells and tissues, and to analyze the correlation among survivin, VEGF-C and lymphatic metastasis. Methods: Plasmid with survivin and VEGF-C shRNA and lentivirus with survivin gene were constructed and transfected into breast cancer cell ZR-75-30. Then the expressions of the two genes were examined using western blot analysis and real-time PCR. The change of invasiveness of breast cancer cells was assessed using matrigel invasion assay. Using immunohistochemistry, the expression of survivin and VEGF-C were analyzed in 108 clinical breast cancer cases with breast cancer tissue and lymph node. Results: Survivin regulated the expression of VEGF-C at both protein and mRNA levels in breast cancer cells. Immunohistochemical analysis showed that the level of VEGF-C expression was significantly related with that of survivin in breast cancer tissues (p0.05). VEGF-C was found to participate in the process of breast cancer cells invasion mediated by survivin. The co-expression of the two and the single expression of any one took significant difference in positive lymph node (p0.05). Conclusions: Survivin takes an important part in regulating the expression of VEGF-C. VEGF-C c