S_8型大孔吸附树脂纯化泽兰总黄酮_酚酸_的工艺研究.pdf

·专 栏 · ·中药与天然药 · S-8 型大孔吸附树脂纯化泽兰总黄酮(酚酸) 的工艺研究 1 1 2 2 2 (1.广州中医药大学第一附属医院，广州 510405；2.广州中医药大学，广州 510405) 摘要：目的 S-8 ()方法 () -()HPLC 结果 S-8 ()0.1 − 1 − 1 g·mL 10 g 7 g 1 mL·min 60 mL80 mL70% 2 mL·min− 1 − 1 95% 1 moL·L 5 () 75.56% 104.59% 1~5 89%()3.99 4.69 结论 S-8 () 关键词() 中图分类号R284.2 文献标志码B 文章编号1007-7693(2010)08-0694-05 Study on Purification of the Total Flavonoids (Phenolic Acids) from Herba Lycopi with S-8 Macroporous Adsorption Resin 1 1 2 2 2 HUANG Yuechun , HUANG Yinghua , LIU Cuiling , WEI Gang , LIU Donghui (1.The Frist Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China; 2.Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China) ABSTRACT: OBJECTIVE To study the technologic conditions of purifying the total flavonoids (phenolic acids) from Herba Lycopi by S-8 resin. METHODS UV-VIS spectrophotometry was used to determine the total flavonoids (phenolic acids), HPLC was used to determine caffeic acid and analyze the characteristic peaks of fingerprint. RESULTS The results showed that the effect was perfect when the concentration of original solution of the extract of Herba Lycopi was 0.1 g·mL− 1, the loading amount was 7 g Herba Lycopi per 10 g dry S-8 resin, the adsorption velocity was 1 mL·min-1, the volume of water was 60 mL, the eluant was 80 mL 70% alcohol, and the elution velocity was 2 mL·min− 1. AB-8 resin could be used at least 5 times repeatedly after being r