大孔树脂纯化核桃隔膜总黄酮的工艺研究_王国军.pdf

·2688 · 中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 19 期 2013 年 10 月 大孔树脂纯化核桃隔膜总黄酮的工艺研究 1 1* 2* 1 1 2 1 王国军 ，唐 辉 ，张淑兰 ，冯 荣 ，王 幻 ，关 丽 ，刘怡娟 1. 石河子大学药学院，新疆 石河子 832000 2. 新疆生产建设兵团医院 药剂科，新疆 乌鲁木齐 830002 摘 要：目的 研究核桃隔膜总黄酮的大孔树脂纯化工艺。方法 通过对 12 种不同型号大孔树脂进行静态吸附与解吸实验， 优选适宜的大孔树脂，并优化分离纯化条件。结果 AB-8 型大孔树脂对核桃隔膜总黄酮有较好的吸附和洗脱效果，其最佳 分离纯化条件为 pH 4.70 ，0.713 5 mg/mL 的质量浓度上样，树脂的上样量为4 mL/g ，上样体积流量为 1.5 BV/h，依次用2 BV 水洗脱，2.5 BV 50% 乙醇洗脱。经AB-8 树脂处理后的总黄酮质量分数达 72.25%，收率为 93.94% 。结论 AB-8 型大孔树脂 用于富集核桃隔膜总黄酮效果最佳，是一种理想的分离纯化介质。 关键词：核桃隔膜；总黄酮；大孔树脂；纯化工艺；吸附动力学特性 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2013)19 - 2688 - 05 DOI: 10.7501/j.issn.0253-2670.2013.19.011 Purification of total flavonoids from diaphragma of Juglans regia by macroporous resin 1 1 2 1 1 2 1 WANG Guo-jun , TANG Hui , ZHANG Shu-lan , FENG Rong , WANG Huan , GUAN Li , LIU Yi-juan 1. College of Pharmacy, Shihezi University, Shihezi 832000, China 2. Department of Pharmacy, Hospital of Xinjiang Production and Construction Corps, Urumqi 830002, China Abstract: Objective To investigate the purification technology of total flavonoids from the diaphragma of Juglans regia by macroporous resin. Methods Twelve macroporous resins were chosn with static and dynamic adsorption and desorption experiments to optimize the purification parameters. Results AB-8 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH value of 4.70, sample mass concentration of 0.713 5 mg/mL, the loaded amount of 4 mL/g, and loading flow rate of 1.5 BV/h. The sample was eluted by water with 2 BV and 50% ethanol of 2.5 BV, respectively. The purity of total flavonoids increased to 72.25% after the purification, and the yield was 93.94%.