孟鲁司特联合沙美特罗替卡松对支气管哮喘患者Th17细胞相关细胞因子表达影响.doc

孟鲁司特联合沙美特罗替卡松对支气管哮喘患者Th17细胞相关细胞因子表达影响 　　[摘要]目的 ?^察口服孟鲁司特联合沙美特罗替卡松吸入治疗对支气管哮喘患者体内辅助性T（Th）17细胞相关细胞因子转化生长因子-β（TGF-β）、白细胞介素（IL）-17和IL-23表达的影响。方法 将2014年10月～2016年4月在本院住院及门诊就诊诊断为支气管哮喘的60例患者按照不同的治疗方法分为常规治疗组20例和孟鲁司特联合沙美特罗替卡松组40例，同时纳入健康对照组20例。分别于入院或门诊就诊治疗后3个月采用ELISA法检测所有患者空腹血清及痰培养上清中Th17细胞相关细胞因子TGF-β、IL-17和IL-23表达的变化。结果 与健康对照组比较，哮喘患者血清及痰培养上清中TGF-β、IL-17和IL-23表达显著升高，差异有统计学意义（P0.05）。结论 Th17细胞相关细胞因子参与哮喘的发病；孟鲁司特联合沙美特罗替卡松治疗可显著降低支气管哮喘患者Th17细胞相关细胞因子的表达。 　　[关键词]支气管哮喘；Th17细胞相关细胞因子；孟鲁司特；沙美特罗替卡松 　　[中图分类号] R562.2+5 [文献标识码] A [文章编号] 1674-4721（2016）11（c）-0027-04 　　[Abstract]Objective To observe effect of oral Montelukast combined with Salmeterol Fluticasone inhalation on experssion levels of cytokines related to T helper （Th） 17 cells transforming growth factor beta （TGF- beta），interleukin （IL）-17 and IL-23 in patients with new-onset bronchial asthma.Methods According to different treatment methods， sixty patient with new-onset bronchial asthma admitted into our hospital from October 2014 to April 2016 were divided into routine treatment group（n=20） and Montelukast combined with Salmeterol Fluticasone group whom received Montelukast combined with Salmeterol Fluticasone （n=40）.Another 20 health people were selected as healthy group.The serum and sputum supernatan levels of inflammatory factors such as TGF-β，IL-17 and IL-23 were measured by ELISA before and after outpatient treatment for 3 months.Results Compared with healthy group，the serum and sputum supernatan levels of inflammatory factors such as TGF-β，IL-17 and IL-23 was higher that in the routine treatment group and the Montelukast combined with Salmeterol Fluticasone group，and the difference was statistically significant （P0.05）.Conclusion Cytokines related cytokines Th17 cells may play important roles in the development of asthma，and using of Montelukast combined with Salmeterol Fluticasone can inhibit the experssion of cytokines related to Th17 in patients with asthma. 　　[Key words]Bronchial asthma；Cytokines related to Th17 c