deep insight into the ganoderma lucidum by comprehensive analysis of its transcriptome灵芝的,深层次的洞察其转录组的综合分析.pdf

Deep Insight into the Ganoderma lucidum by Comprehensive Analysis of Its Transcriptome 1 1 1 1 1 1 1 Guo-Jun Yu , Man Wang , Jie Huang , Ya-Lin Yin , Yi-Jie Chen , Shuai Jiang , Yan-Xia Jin , 1 1 1,2 1,3,4 Xian-Qing Lan , Barry Hon Cheung Wong , Yi Liang , Hui Sun * 1 State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China, 2 Department of Clinical Immunology, Guangdong Medical College, Dongguan, People’s Republic of China, 3 Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, People’s Republic of China, 4 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan, People’s Republic of China Abstract Background: Ganoderma lucidum is a basidiomycete white rot fungus and is of medicinal importance in China, Japan and other countries in the Asiatic region. To date, much research has been performed in identifying the medicinal ingredients in Ganoderma lucidum. Despite its important therapeutic effects in disease, little is known about Ganoderma lucidum at the genomic level. In order to gain a molecular understanding of this fungus, we utilized Illumina high-throughput technology to sequence and analyze the transcriptome of Ganoderma lucidum. Methodology/Principal Findings: We obtained 6,439,690 and 6,416,670 high-quality reads from the mycelium and fruiting body of Ganoderma lucidum, and these were assembled to form 18,892 and 27,408 unigenes, respectively. A similarity search was performed against the NCBI non-redundant nucleotide database and a customized dat