biochemical properties and potential applications of recombinant leucine aminopeptidase from bacillus kaustophilus ccrc 11223的生化性质和潜在的应用重组亮氨酸氨基肽酶芽孢杆菌kaustophilus ccrc 11223.pdf

Int. J. Mol. Sci. 2011, 12, 7609-7625; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Biochemical Properties and Potential Applications of Recombinant Leucine Aminopeptidase from Bacillus kaustophilus CCRC 11223 Yanfei Shen 1,†, Fanghua Wang 2,†, Dongming Lan 3, Yuanyuan Liu 2, Bo Yang 1,* and Yonghua Wang 2,* 1 Department of Biotechnology, School of Bioscience Bioengineering, South China University of Technology, Guangzhou 510006, Guangdong, China; E-Mail: petsyf@126.com 2 College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, Guangdong, China; E-Mails: sdzbwfh@126.com (F.W.); lyy0537@163.com (Y.L.) 3 School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China; E-Mail: landongming@ † These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: yangbo@ (B.Y.); yonghw@ (Y.W.); Tel./Fax: +86-0-20-3938-0696 (B.Y.); +86-0-20-8711-3842 (Y.W.). Received: 27 September 2011; in revised form: 25 October 2011 / Accepted: 28 October 2011 / Published: 7 November 2011 Abstract: Experiments were carried out to investigate the effects of various factors on the activity and conformation of recombinant leucine aminopeptidase of Bacillus kaustophilus CCRC 11223 (BkLAP) and potential utilization of BkLAP in the hydrolys