biochemical properties and potential applications of recombinant leucine aminopeptidase from bacillus kaustophilus ccrc 11223的生化性质和潜在的应用重组亮氨酸氨基肽酶芽孢杆菌kaustophilus ccrc 11223.pdf
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Int. J. Mol. Sci. 2011, 12, 7609-7625; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Biochemical Properties and Potential Applications of
Recombinant Leucine Aminopeptidase from Bacillus
kaustophilus CCRC 11223
Yanfei Shen 1,†, Fanghua Wang 2,†, Dongming Lan 3, Yuanyuan Liu 2, Bo Yang 1,* and
Yonghua Wang 2,*
1
Department of Biotechnology, School of Bioscience Bioengineering, South China University of
Technology, Guangzhou 510006, Guangdong, China; E-Mail: petsyf@126.com
2
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou
510640, Guangdong, China; E-Mails: sdzbwfh@126.com (F.W.); lyy0537@163.com (Y.L.)
3 School of Chemistry and Chemical Engineering, South China University of Technology,
Guangzhou 510640, Guangdong, China; E-Mail: landongming@
† These authors contributed equally to this work.
* Authors to whom correspondence should be addressed; E-Mails: yangbo@ (B.Y.);
yonghw@ (Y.W.); Tel./Fax: +86-0-20-3938-0696 (B.Y.); +86-0-20-8711-3842 (Y.W.).
Received: 27 September 2011; in revised form: 25 October 2011 / Accepted: 28 October 2011 /
Published: 7 November 2011
Abstract: Experiments were carried out to investigate the effects of various factors on the
activity and conformation of recombinant leucine aminopeptidase of Bacillus kaustophilus
CCRC 11223 (BkLAP) and potential utilization of BkLAP in the hydrolys
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